{"id":4772,"date":"2021-06-07T13:43:38","date_gmt":"2021-06-07T11:43:38","guid":{"rendered":"https:\/\/cobomation.de\/droplet-based-high-throughput-screening\/"},"modified":"2021-10-08T20:55:10","modified_gmt":"2021-10-08T18:55:10","slug":"droplet-based-high-throughput-screening","status":"publish","type":"post","link":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/","title":{"rendered":"Droplet-based high-throughput screening has never been easier"},"content":{"rendered":"[vc_row type=&#8221;in_container&#8221; full_screen_row_position=&#8221;middle&#8221; column_margin=&#8221;default&#8221; column_direction=&#8221;default&#8221; column_direction_tablet=&#8221;default&#8221; column_direction_phone=&#8221;default&#8221; scene_position=&#8221;center&#8221; text_color=&#8221;dark&#8221; text_align=&#8221;left&#8221; row_border_radius=&#8221;none&#8221; row_border_radius_applies=&#8221;bg&#8221; overlay_strength=&#8221;0.3&#8243; gradient_direction=&#8221;left_to_right&#8221; shape_divider_position=&#8221;bottom&#8221; bg_image_animation=&#8221;none&#8221;][vc_column column_padding=&#8221;no-extra-padding&#8221; column_padding_tablet=&#8221;inherit&#8221; column_padding_phone=&#8221;inherit&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; column_link_target=&#8221;_self&#8221; gradient_direction=&#8221;left_to_right&#8221; overlay_strength=&#8221;0.3&#8243; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; tablet_text_alignment=&#8221;default&#8221; phone_text_alignment=&#8221;default&#8221; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][vc_column_text]Postek et al. demonstrated an automated high-throughput screening for MIC determination (minimal inhibitory concentration) of an antibiotic in nanolitre droplets for different E.coli concentrations. With the help of the specially developed DropChop chip, different physically separated compartments can be generated. With the resulting ultra-small sample volumes, comprehensive test series can subsequently be carried out with maximum efficiency and large scaling.<\/p>\n<h3>Laboratory Setup<\/h3>\n<p>In this study, \u00b5l-volume droplets of different E.coli dilutions were automatically generated by neMESYS syringe pumps in combination with the versatile rotAXYS miniature positioning system. These \u00b5l-volume droplets were then transferred into nL-volume droplets within a compartment using the DropChop chip, which was specially developed for this specific application, then cultured and subsequently comprehensively analyzed using fluorescence microscopy.<\/p>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4498 size-full\" src=\"https:\/\/cetoni.de\/wp-content\/uploads\/20190717_Anwendung_kaminski_neu.png\" alt=\"\" width=\"1280\" height=\"835\" srcset=\"https:\/\/cetoni.com\/wp-content\/uploads\/20190717_Anwendung_kaminski_neu.png 1280w, https:\/\/cetoni.com\/wp-content\/uploads\/20190717_Anwendung_kaminski_neu-300x196.png 300w, https:\/\/cetoni.com\/wp-content\/uploads\/20190717_Anwendung_kaminski_neu-1024x668.png 1024w, https:\/\/cetoni.com\/wp-content\/uploads\/20190717_Anwendung_kaminski_neu-768x501.png 768w\" sizes=\"auto, (max-width: 1280px) 100vw, 1280px\" \/><\/h3>\n<p>This application illustrates the advantages of the CETONI product philosophy: Thanks to the modular system design, various devices could be combined effortlessly and integrated easily into the customer&#8217;s software environment via the practical CETONI software development kit. The precisely controllable rotAXYS facilitated the fully automated and precise processing of \u00b5l-volume droplets by the neMESYS syringe pump system from a 96-well microtiter plate. The reproducible generation of these uniform small droplets was mainly achieved by the interruption-free motion characteristics of the neMESYS syringe pumps, while the physical separation of subsequent emulsions was achieved with the easy programming of the pump protocol of operations with the Qmix software.<\/p>\n<p>The CETONI system also easily met the requirements of a sterile working environment with the right accessories.<\/p>\n<h3>Used CETONI devices:<\/h3>\n<ul>\n<li>Base 120<\/li>\n<li>3 x Low Pressure Syringe Pump neMESYS 290N<\/li>\n<li>Compact Positioning System rotAXYS<\/li>\n<\/ul>\n<h3>Literature:<\/h3>\n<p>Postek W., Gargulinski P., Scheler O., Kaminski T. , Garstecki P. Microfluidic screening of antibiotic susceptibility at a single-cell level shows the inoculum effect of cefotaxime on E. coli[\/vc_column_text][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"<p>[vc_row type=&#8221;in_container&#8221; full_screen_row_position=&#8221;middle&#8221; column_margin=&#8221;default&#8221; column_direction=&#8221;default&#8221; column_direction_tablet=&#8221;default&#8221; column_direction_phone=&#8221;default&#8221; scene_position=&#8221;center&#8221; text_color=&#8221;dark&#8221; text_align=&#8221;left&#8221; row_border_radius=&#8221;none&#8221; row_border_radius_applies=&#8221;bg&#8221; overlay_strength=&#8221;0.3&#8243; gradient_direction=&#8221;left_to_right&#8221; shape_divider_position=&#8221;bottom&#8221; bg_image_animation=&#8221;none&#8221;][vc_column column_padding=&#8221;no-extra-padding&#8221; column_padding_tablet=&#8221;inherit&#8221; column_padding_phone=&#8221;inherit&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; column_link_target=&#8221;_self&#8221; gradient_direction=&#8221;left_to_right&#8221; overlay_strength=&#8221;0.3&#8243; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; tablet_text_alignment=&#8221;default&#8221;&#8230;<\/p>\n","protected":false},"author":1,"featured_media":4500,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[98,96],"class_list":{"0":"post-4772","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-publication","8":"tag-cetoniacademy-en","9":"tag-publication"},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Droplet-based high-throughput screening has never been easier -<\/title>\n<meta name=\"description\" content=\"Postek et al. nicely demonstrated an automated high-throughput screening for MIC determination (minimal inhibitory concentration)\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Droplet-based high-throughput screening has never been easier -\" \/>\n<meta property=\"og:description\" content=\"Postek et al. nicely demonstrated an automated high-throughput screening for MIC determination (minimal inhibitory concentration)\" \/>\n<meta property=\"og:url\" content=\"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/\" \/>\n<meta property=\"og:site_name\" content=\"CETONI GmbH\" \/>\n<meta property=\"article:published_time\" content=\"2021-06-07T11:43:38+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-10-08T18:55:10+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/cetoni.com\/wp-content\/uploads\/header_kaminski.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1280\" \/>\n\t<meta property=\"og:image:height\" content=\"380\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"CETONI\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"CETONI\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/\"},\"author\":{\"name\":\"CETONI\",\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/#\\\/schema\\\/person\\\/f2d4de804c915072039aae63b057de8e\"},\"headline\":\"Droplet-based high-throughput screening has never been easier\",\"datePublished\":\"2021-06-07T11:43:38+00:00\",\"dateModified\":\"2021-10-08T18:55:10+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/\"},\"wordCount\":431,\"image\":{\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/cetoni.com\\\/wp-content\\\/uploads\\\/header_kaminski.png\",\"keywords\":[\"#CETONIacademy\",\"Publication\"],\"articleSection\":[\"Publication\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/\",\"url\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/\",\"name\":\"Droplet-based high-throughput screening has never been easier -\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/cetoni.com\\\/wp-content\\\/uploads\\\/header_kaminski.png\",\"datePublished\":\"2021-06-07T11:43:38+00:00\",\"dateModified\":\"2021-10-08T18:55:10+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/#\\\/schema\\\/person\\\/f2d4de804c915072039aae63b057de8e\"},\"description\":\"Postek et al. nicely demonstrated an automated high-throughput screening for MIC determination (minimal inhibitory concentration)\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/#primaryimage\",\"url\":\"https:\\\/\\\/cetoni.com\\\/wp-content\\\/uploads\\\/header_kaminski.png\",\"contentUrl\":\"https:\\\/\\\/cetoni.com\\\/wp-content\\\/uploads\\\/header_kaminski.png\",\"width\":1280,\"height\":380,\"caption\":\"Mikrofluidiksystem SpritzenpumpeTropfenbasiertes Hochdurchsatz-Screening\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/droplet-based-high-throughput-screening\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Startseite\",\"item\":\"https:\\\/\\\/cetoni.com\\\/en\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Droplet-based high-throughput screening has never been easier\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/#website\",\"url\":\"https:\\\/\\\/cetoni.com\\\/en\\\/\",\"name\":\"CETONI GmbH\",\"description\":\"Automatisierungs- und Mikrofluidikl\u00f6sungen f\u00fcr das Labor\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/cetoni.com\\\/en\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/cetoni.com\\\/en\\\/#\\\/schema\\\/person\\\/f2d4de804c915072039aae63b057de8e\",\"name\":\"CETONI\",\"sameAs\":[\"http:\\\/\\\/cobomation.de\"],\"url\":\"https:\\\/\\\/cetoni.com\\\/en\\\/author\\\/cetoni\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Droplet-based high-throughput screening has never been easier -","description":"Postek et al. nicely demonstrated an automated high-throughput screening for MIC determination (minimal inhibitory concentration)","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/","og_locale":"en_US","og_type":"article","og_title":"Droplet-based high-throughput screening has never been easier -","og_description":"Postek et al. nicely demonstrated an automated high-throughput screening for MIC determination (minimal inhibitory concentration)","og_url":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/","og_site_name":"CETONI GmbH","article_published_time":"2021-06-07T11:43:38+00:00","article_modified_time":"2021-10-08T18:55:10+00:00","og_image":[{"width":1280,"height":380,"url":"https:\/\/cetoni.com\/wp-content\/uploads\/header_kaminski.png","type":"image\/png"}],"author":"CETONI","twitter_card":"summary_large_image","twitter_misc":{"Written by":"CETONI","Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/#article","isPartOf":{"@id":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/"},"author":{"name":"CETONI","@id":"https:\/\/cetoni.com\/en\/#\/schema\/person\/f2d4de804c915072039aae63b057de8e"},"headline":"Droplet-based high-throughput screening has never been easier","datePublished":"2021-06-07T11:43:38+00:00","dateModified":"2021-10-08T18:55:10+00:00","mainEntityOfPage":{"@id":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/"},"wordCount":431,"image":{"@id":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/#primaryimage"},"thumbnailUrl":"https:\/\/cetoni.com\/wp-content\/uploads\/header_kaminski.png","keywords":["#CETONIacademy","Publication"],"articleSection":["Publication"],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/","url":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/","name":"Droplet-based high-throughput screening has never been easier -","isPartOf":{"@id":"https:\/\/cetoni.com\/en\/#website"},"primaryImageOfPage":{"@id":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/#primaryimage"},"image":{"@id":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/#primaryimage"},"thumbnailUrl":"https:\/\/cetoni.com\/wp-content\/uploads\/header_kaminski.png","datePublished":"2021-06-07T11:43:38+00:00","dateModified":"2021-10-08T18:55:10+00:00","author":{"@id":"https:\/\/cetoni.com\/en\/#\/schema\/person\/f2d4de804c915072039aae63b057de8e"},"description":"Postek et al. nicely demonstrated an automated high-throughput screening for MIC determination (minimal inhibitory concentration)","breadcrumb":{"@id":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/#primaryimage","url":"https:\/\/cetoni.com\/wp-content\/uploads\/header_kaminski.png","contentUrl":"https:\/\/cetoni.com\/wp-content\/uploads\/header_kaminski.png","width":1280,"height":380,"caption":"Mikrofluidiksystem SpritzenpumpeTropfenbasiertes Hochdurchsatz-Screening"},{"@type":"BreadcrumbList","@id":"https:\/\/cetoni.com\/en\/droplet-based-high-throughput-screening\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Startseite","item":"https:\/\/cetoni.com\/en\/"},{"@type":"ListItem","position":2,"name":"Droplet-based high-throughput screening has never been easier"}]},{"@type":"WebSite","@id":"https:\/\/cetoni.com\/en\/#website","url":"https:\/\/cetoni.com\/en\/","name":"CETONI GmbH","description":"Automatisierungs- und Mikrofluidikl\u00f6sungen f\u00fcr das Labor","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/cetoni.com\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/cetoni.com\/en\/#\/schema\/person\/f2d4de804c915072039aae63b057de8e","name":"CETONI","sameAs":["http:\/\/cobomation.de"],"url":"https:\/\/cetoni.com\/en\/author\/cetoni\/"}]}},"_links":{"self":[{"href":"https:\/\/cetoni.com\/en\/wp-json\/wp\/v2\/posts\/4772","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cetoni.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cetoni.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cetoni.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cetoni.com\/en\/wp-json\/wp\/v2\/comments?post=4772"}],"version-history":[{"count":0,"href":"https:\/\/cetoni.com\/en\/wp-json\/wp\/v2\/posts\/4772\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cetoni.com\/en\/wp-json\/wp\/v2\/media\/4500"}],"wp:attachment":[{"href":"https:\/\/cetoni.com\/en\/wp-json\/wp\/v2\/media?parent=4772"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cetoni.com\/en\/wp-json\/wp\/v2\/categories?post=4772"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cetoni.com\/en\/wp-json\/wp\/v2\/tags?post=4772"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}